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Figure 2 | BMC Plant Biology

Figure 2

From: Cell wall traits as potential resources to improve resistance of durum wheat against Fusarium graminearum

Figure 2

Monosaccharide compositions and immunodot analysis of cell wall polysaccharides in spikes of 02-5B-318 R and Saragolla S plants. (a) Cellulose-derived glucose, (b) Fucose (Fuc), rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), xylose (Xyl), galacturonic acid (Gal A) and glucuronic acid (Glu A) released after 2 M TFA hydrolysis were determined by using a high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) system, (c) Arabinose/Xylose ratio in spikes of 02-5B-318R and SaragollaS. Results represent the mean ± SD of three replicates (n = 6). Asterisks indicate data sets significantly different between 02-5B-318R and SaragollaS according to Student’s t-test (p < 0.001). (d) Immunodot analysis for xylan substitution using LM11 antibody. The micrograms of in KOH 1 M hemicellulose fraction from the two genotypes were applied to the nitrocellulose membrane were indicated. The experiments were repeated three times with similar results. R = 02-5B-318R; S = SaragollaS.

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