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Table 2 Kinetic parameters of poplar GS recombinant enzymes

From: Redundancy and metabolic function of the glutamine synthetase gene family in poplar

 

Ammonium

Glutamate

ATP

GS1.1

K m  = 5 μM

Negative cooperativity

K m  = 1.4 mM

  

nH = 0.6

 
 

K cat/ K m  = 1.2 x107 a

  

GS1.2

   

819912

K m  = 200 mM

Negative cooperativity

K m  = 0.9 mM

716066

K m  = 190 mM

nH = 0.6

K m  = 1.0 mM

 

K cat/ K m  = 0.8 x106 a

  

GS1.3

K m  = 110 μM

Negative cooperativity

K m  = 1.0 mM

  

nH = 0.6

 
 

K cat/ K m  = 1.1 x106 a

  

GS2

Positive cooperativity

K m  = 26 mM

K m  = 0.7 mM

 

nH = 1.7; S 0.5  = 0.3

  
  1. The biosynthetic assay was used [39,40].
  2. aM−1 s−1.