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Table 2 Kinetic parameters of poplar GS recombinant enzymes

From: Redundancy and metabolic function of the glutamine synthetase gene family in poplar

  Ammonium Glutamate ATP
GS1.1 K m  = 5 μM Negative cooperativity K m  = 1.4 mM
   nH = 0.6  
  K cat/ K m  = 1.2 x107 a   
GS1.2    
819912 K m  = 200 mM Negative cooperativity K m  = 0.9 mM
716066 K m  = 190 mM nH = 0.6 K m  = 1.0 mM
  K cat/ K m  = 0.8 x106 a   
GS1.3 K m  = 110 μM Negative cooperativity K m  = 1.0 mM
   nH = 0.6  
  K cat/ K m  = 1.1 x106 a   
GS2 Positive cooperativity K m  = 26 mM K m  = 0.7 mM
  nH = 1.7; S 0.5  = 0.3   
  1. The biosynthetic assay was used [39,40].
  2. aM−1 s−1.