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Figure 6 | BMC Plant Biology

Figure 6

From: Redundancy and metabolic function of the glutamine synthetase gene family in poplar

Figure 6

Cell and tissue distribution of poplar GS expression examined by LCM and qPCR analysis. Tissue sections from poplar leaves, stems and roots were processed for LCM and total RNA isolated as described in Methods. Three independent biological replicates were processed. The expression levels of duplicated genes PtGS2, PtGS1.1, PtGS1.2 and PtGS1.3 were determined by qPCR using oligonucleotide primers designed to amplify the transcripts encoded by each pair of genes [16]. Leaf: L, Lamina; V, vascular cells; P, parenchyma. Stem: P, pith; X, xylem; F, phloem; CP cortical parenchyma, Root: CR, cortical root; VC, vascular cylinder. Higher magnification shows the presence of numerous plastids in the cortical cells. Each value represents the mean ± SD of 3 biological replicates. Statistics analysis were performed by Anova and the significative diferences were calculated by Tukey’s t test (p < 0.01).

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