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Figure 3 | BMC Plant Biology

Figure 3

From: Redundancy and metabolic function of the glutamine synthetase gene family in poplar

Figure 3

Effect of temperature and pH in the activity of poplar GS holoenzymes. a) The activity of purified native isoenzymes were determined at different temperatures from 10°C to 70°C. The maximum level (100%) of activities for GS1.1, GS1.2, GS1.3 and GS2 were 23 nkatal, 65 nkatal, 50 nkatal and 34 nkatal, respectively. Values are the mean ± SD of at least three independent determinations. b) The activity of purified native isoenzymes were determined at different pH values. The maximum level (100%) of activity for GS1.1, GS1.2, GS1.3 and GS2 were 38 nkatal, 56 nkatal, 48 nkatal and 25 nkatal, respectively. The following buffers were used: Acetate (4.5 ), Mes (5.5-6.5 ), ▲ MOPS (6.6-8), Tris (8–9), □ Sodium carbonate (10). Values are the mean ± SD of at least three independent determinations.

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