Figure 1
From: Redundancy and metabolic function of the glutamine synthetase gene family in poplar
Recombinant overproduction of poplar glutamine synthetases. a) Schematic representation of constructs made with Populus trichocharpa FLcDNA from N- (left) to C-(right) terminals. White rectangle: FLcDNA; black rectangle: His-tag. b) Expression of poplar GS2, GS1.1, GS1.2 and GS1.3 isoenzymes in E. coli. Levels of GS activity for each isoenzyme are shown in the upper part of the Figure. The maximum level (100%) of activity was 67 nkatals. Values are the mean ± SD of at least three independent determinations. Immunoblot of the same protein extracts is shown in the lower part of the Figure. c) Electrophoretic analysis of the homogenous preparations of poplar GS2, GS1.1, GS1.2 and GS1.3 isoenzymes. Molecular markers were loaded on the left.