Summary of sequential steps adapted to study evolution of AGOs in plants. A total of 263 AGO sequences from 37 plant species were used in this analysis. Additionally, 5 AGOs from T. castaneum, and 1 each from Human (PDB code 4F3T) and K. polysporus (PDB code 4F1N) were also used (as out groups) to create the ‘AGO dataset I’, comprising a total of 270 AGO sequences. The list of AGOs for each species is available in Additional file 1. After MSA and trimming of poorly aligned regions or large gaps, ‘AGO dataset II’ was generated to contain 270 sequences (rows) and 620 positions (columns; Additional file 2).