Clustered heat-map of the metabolite changes related to developmental phases. The data were derived from three independent experiments. In each experiment, the samples were collected from a group of nine plants in three replicates except for the mvp crowns during the C and Co stages, in which the samples could be collected only in two experiments. Only those compounds that showed significant changes (one-way MANOVA, P < 0.05) between the control and cold-treated samples (Co/C), cold-treated samples in the vegetative and double ridge formation stages (Dr/Co) and double ridge formation via spikelet formation stages (Sp/Dr) in all of the experiments are included in the heat-map. The red colour depicts increases, while the green colour depicts decreases from one stage to another (A) and from a given stage to the control stage (B) in concentrations of the compounds that are listed in the figure. The scale is in log2. 5-Oxo-proline was derived from glutamic acid during the methoximation step. CS, Chinese Spring; CS(C) Chinese Spring with Cheyenne chromosome 5A substitution; CS(T), Chinese Spring with Triticum spelta chromosome 5A substitution; Tm, Triticum monococcum; mvp, mvp-2 mutant of Triticum monococcum; C, control; Co, after cold treatment but still in vegetative phase; Dr: double ridge formation stage; Sp, spikelet formation stage.