qRT-PCR analysis of
genes across developmental stages. The values represent means ± SD of nine plants in three parallels. Asterisks indicate values determined by t test to be significantly different from the C stage in each individual line (P < 0.01). CS, Chinese Spring; CS(Ch5A) Chinese Spring with Cheyenne chromosome 5A substitution; CS(Tsp5A), Chinese Spring with Triticum spelta chromosome 5A substitution; Tm, Triticum monococcum; mvp, mvp-2 mutant of Triticum monococcum; C, control; Co, after cold treatment but still in vegetative phase; Dr: double ridge formation stage; Sp, spikelet formation stage; Sh, shoot; Cr, crown.