Figure 5

DNA dsb basal levels and repair kinetics are altered in the atrad21.1 and atrad21.3 mutants. (A) atrad21.1 and atrad21.3 single mutants’ DNA dsb damage repair kinetics is similar. During the first 60 minutes after DNA dsb damage induction, atrad21.1 and atrad21.3 mutants retain more unrepaired DNA dsb than Col. This difference is more striking at 10 minutes (62.1% to 72.2% of induced DNA dsb remain unrepaired in the single mutants versus 40.2% in Col), 20 minutes (55.4% to 60.9% in the single mutant versus 31.3% in Col) and 60 minutes (20.3% to 22.1% in the single mutants versus 17.1% in Col) after DNA dsb damage induction. The atrad21.1 atrad21.3 double mutant has a Col-like DNA dsb damage repair kinetics. DNA dsb damage quantification was carried out on nuclei from 10-days old seedlings harvested 0, 3, 5, 10, 20, 60 and 180 minutes after exposure to 30 μg/ml Bleomycin. Col - wild-type Columbia-0 plant. (B) atrad21.1, atrad21.3 and atrad21.1 atrad21.3 mutants have higher basal levels of DNA dsb than Col. The amount of DNA dsb detected, by comet assay, in nuclei obtained from seedlings not exposed to DNA dsb inducing agent, indicates that the amount of DNA dsb detected in Col is significantly lower than the amount detected in atrad21.1, atrad21.3 and atrad21.1 atrad21.3 mutants. DNA dsb damage quantification was carried out on nuclei from 10-days old seedlings that were not exposed to Bleomycin. Error bars represent the standard error. Col - wild-type Columbia-0 plant. Comet assay data is available in Additional file 1: Table S6.