Figure 5

Flower color after overexpression of CsF3′5′H and qRT-PCR of transgenic tobacco plants. (A) Tobacco flowers of wild-type (CK) and CsF3′5′H transgenes (Line 1). (B) Tobacco flowers of wild-type (CK), CsF3′5′H transgenes (Line 1, 3, 9 and 15)and qRT-PCR for CsF3′5′H in flowers from CK and transgenic lines. (C) HPLC chromatograms of anthocyanidins (at 530 nm) and flavonol glacosides (at 340 nm) in tobacco flowers from CK and Line 1 (1: DEL; 2: CYA; 3: quercetin-3-O-rutinoside, 4: kaempferol-3-O-rutinoside). (D) Concentration of anthocyanidins in tobacco flowers from CK, CsF3′5′H transgenes (Line 1, 3 9, and 15) and vector control. The data represent the mean ± SD from three independent measurements. (E) qRT-PCR for flavonoid-related genes in tobacco flowers from CK, CsF3′5′H transgenes (Line 1, Line3 Line 9) and vector control. *indicated the significant level at P < 0.05. ^#indicated the significant level compared between every detected lines versus CK (wild type and vector control).