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Figure 4 | BMC Plant Biology

Figure 4

From: Functional analysis of Flavonoid 3′,5′-hydroxylase from Tea plant (Camellia sinensis): critical role in the accumulation of catechins

Figure 4

HPLC chromatograms of flavanones or dihydroflavonols formation in CsF3′5′H assays with tea leaf enzyme. (A) Reaction assay with substrate N of heat-denatured protein in the control treatment (the crude enzyme extract were heated to 100°C to inactivate enzyme activities); (B) Reaction assay with substrate N of the crude enzyme extract from the leave of tea; (C) Reaction assay with substrate DHQ of heat-denatured protein in the control treatment; (D) Reaction assay with substrate DHQ of the crude enzyme extract from the leave of tea.

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