Figure 4From: Functional analysis of Flavonoid 3′,5′-hydroxylase from Tea plant (Camellia sinensis): critical role in the accumulation of catechinsHPLC chromatograms of flavanones or dihydroflavonols formation in CsF3′5′H assays with tea leaf enzyme. (A) Reaction assay with substrate N of heat-denatured protein in the control treatment (the crude enzyme extract were heated to 100°C to inactivate enzyme activities); (B) Reaction assay with substrate N of the crude enzyme extract from the leave of tea; (C) Reaction assay with substrate DHQ of heat-denatured protein in the control treatment; (D) Reaction assay with substrate DHQ of the crude enzyme extract from the leave of tea.Back to article page