Figure 3

Representative 24 cm two-dimensional gel electrophoresis (2D-DIGE) image of the proteome of genetically modified maize plant leaves AG8025 hybrid varieties MON-89Ø34-3 and MON-ØØ6Ø3-6 single events, and MON-89Ø34-3 x MON-ØØ6Ø3-6 stacked event, and non-modified maize (conventional counterpart AG8025 hybrid variety and landrace Pixurum 5 variety) grown under controlled conditions. Two random replicate samples were run together with an internal standard sample, each labeled with a different fluorescence. Individualgel images were obtained and plotted together using ImageQuant TL software from GE healthcare. Linear isoelectric focusing pH 4–7 for the first dimension and 12% SDS–PAGE gels in the second dimension were used. Molecular mass standard range from 250 to 10 kDa are given on the left side. Red arrows point to differentially expressed protein spots selected for mass spectrometry identification. ID of identified proteins from Table 2 are indicated in red numbers.