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Table 2 Constructs used for plant expression

From: A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis

Name

Construct

Expression vector

Plant background

p35S:UGE2

p35S:FLAG-AtUGE2

pMDC32

Col-0, uge4, GalS1-OE

p35S:UGE4

p35S:FLAG-AtUGE4

pMDC32

Col-0, uge4

p35S:UGE5

p35S:FLAG-AtUGE5

pMDC32

Col-0, uge4

p35S:UGEc

p35S:FLAG-PtUGEc

pMDC32

Col-0, uge4

p35S:GalS1

p35S:YFP-GalS1

pEarleyGate104

Col-0

pIRX5:NST1-UGE2

pIRX5:NST1-2A-AtUGE2

pTKan-pIRX5-GWR3R2

Col-0, GalS1-OE

EVC

35S:pvu2 (non coding)

pMDC32

Col-0, uge4