Figure 7

MYC_771 and MYC_94937 regulate transcription of JAZ_1405. Transient expression in a single cell system was used to assess the transcriptional effect of MYC_771 and MYC_94937 proteins on JAZ_1405. The fluorescence of the reporter gene was measured by flow cytometry upon co-transfection with a reporter construct (pJAZ_1405::GFP) and an effector construct (35S::HbMYC_771 or 35S::HbMYC94937). The basal fluorescence obtained in the assay transfected with the reporter construct and pJAZ_1405(−267) was taken as the reference (100% relative fluorescence). (A) Schematic representation of the constructs used for transient expression assays. The reporter construct consisted of the JAZ_1405 promoter and GFP coding sequence. The effector constructs expressed HbMYC_771 and HbMYC_94937 under the control of the CaMV 35S promoter. (B)The fluorescence of protoplasts co-transformed with pJAZ_1405::GFP and pMDC32 were used as reference (RFU = 1). Ratio of fluorescence from pJAZ_1405 (−267)::GFP or pJAZ_1405(−955)::GFP with HbMYC_771 or HbMYC_94937 on the reference fluorescence were displayed in RFU. Star indicate a significant difference of fluorescence obtained with the same MYC for long and short promoters. (C) Effect of MYC_771 and MYC_94937 on the JAZ_1405 promoter. The value with the short promoter was set at 1 and relative values are shown. Error bars indicate the standard deviation of results from 6 replicates.