Figure 2

Expression pattern of the jasmonate transduction pathway members in Hevea plantlets in response to abiotic and hormonal stress. The data obtained by quantitative RT-PCR corresponded to the transcript accumulation of genes acting in the JA signalling pathway in response to wounding, dehydration, ethylene and methyl jasmonate (MeJA) in stems of 3-month-old plantlets. RNAs were collected after 1 h, 4 h, 8 h and 24 h of treatment.,Gene expression values were normalized using Rh2B as internal control, and then, ratio between treated and untreated plant have been calculatedfor each time point. A comparison of means test was carried out to compare the transcript abundance in stressed plants and control plants. Significant changes were indicated by “s” above error bars. (A) Expression pattern of jasmonate pathway genes in control plant. (B) Expression pattern of jasmonate pathway genes in response to wounding. Expression pattern of HbERFIXc4and HbERFIXc5 have been tested to validate efficiency of wounding treatment. (C) Expression pattern of jasmonate pathway genes in response to dehydration. Expression pattern of HbERFIV-a3 has been tested to validate efficiency dehydration treatment. (D) Expression pattern of jasmonate pathway genes in response to ethylene. Expression pattern of HbERFIXc4and HbERFIXc5 have been tested to validate efficiency ethylene treatment. (E) Expression pattern of jasmonate pathway genes in response to jasmonate. Expression pattern of HbERFIXc4and HbERFIXc5 have been followed to validate efficiency jasmonate treatment. The data presented correspond to the meanof 3 independent biological replicates.