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Table 4 A list of 18 Competitive Allele-Specific PCR (KASP) markers developed for MAS of PHS from SNPs flanking PHS QTL on chromosomes 3B, 4A, 7B and 7D in a DH population of a RL4452/‘AC Domain’ cross

From: Identification of candidate genes, regions and markers for pre-harvest sprouting resistance in wheat (Triticum aestivum L.)

Sl.

KASP marker

Source SNP

Chr

PHS trait

1.

Kasp3B_wsnp_Ku_rep_c72700_72370664

wsnp_Ku_rep_c72700_72370664

3B

SI

2.

Kasp3B_RAC875_rep_c116515_181,

RAC875_rep_c116515_181,

3B

SI

3.

Kasp3B_BobWhite_rep_c64944_264

BobWhite_rep_c64944_264

3B

SI

4.

Kasp3B_wsnp_Ex_c16378_24870688

wsnp_Ex_c16378_24870688

3B

SI

5.

Kasp3B_RAC875_c530_354

RAC875_c530_354

3B

SI

6.

Kasp4A_BS00072025_51

BS00072025_51

4A

GI, SI, FN

7.

Kasp4A_Kukri_c12563_52

Kukri_c12563_52

4A

GI, SI, FN

8.

Kasp4A_RAC875_c21369_425

RAC875_c21369_425

4A

GI, SI, FN

9.

Kasp4A_wsnp_Ex_c16175_24619793

wsnp_Ex_c16175_24619793

4A

GI, SI, FN

10

Kasp7B_wsnp_Ex_c908_1754208

wsnp_Ex_c908_1754208

7B.1

SI

11

Kasp7B_RFL_Contig124_558

RFL_Contig124_558

7B.1

SI

12

Kasp7B_RAC875_c1638_165

RAC875_c1638_165

7B.1

SI

13

Kasp7B_wsnp_Ex_rep_c69639_68590556

wsnp_Ex_rep_c69639_68590556

7B.1

SI

14

Kasp7B_Ku_c32389_1009

Ku_c32389_1009

7B.1

SI

15

Kasp7D_Excalibur_c22419_460

Excalibur_c22419_460

7D.2

FN

16

Kasp7D_RAC875_c1829_321

RAC875_c1829_321

7D.2

FN

17

Kasp7D_Kukri_c32845_116

Kukri_c32845_116

7D.2

FN

18

Kasp7D_wsnp_CAP8_rep_c9647_4198594

wsnp_CAP8_rep_c9647_4198594

7D.2

FN