Figure 5

The effect of Pi supply on the Po and Pi concentrations in 44-day-old Arabidopsis Col-0 WT and Atpht1 plants grown in hydroponics. The P concentrations in the inorganic (Pi) and organic (Po) pools were determined in roots (A & B) and shoots (C & D) of plants grown on nutrient solution containing a sufficient Pi supply (A & C) or no Pi supply (B & D). Po was calculated as total P minus Pi. Plants were grown in nutrient solution containing 250 μM Pi for 30 d, and then transferred to solution containing 250 μM Pi or no added Pi for 14 d before harvest. For Po and Pi concentrations, values are means ± S.D. (n = 3 biological replicates of 12 plants each grown at separate times). *indicates significantly different means (P <0.05) relative to Col-0 according to one-way ANOVA followed by Tukey’s Multiple Comparison of Means.