Figure 4

Short-term accumulation of Pi by P-depleted 48-day-old Col-0 and Atpht1 knock-out Arabidopsis plants grown in hydroponics. Accumulation of Pi in roots (A) and shoots (B) of WT and pht1 mutant plants over a 6-hour time course. Seedlings were grown in nutrient solution containing 250 μM Pi for 30 d, transferred to solution without Pi for 18 d to deplete plant P pools, and then transferred to solutions containing 250 μM Pi. Tissues were harvested every 30 mins for 6 h after the last transfer. Values are means ± S.D. (n = 3 biological replicates with 12 plants each grown at separate times). Error bars have been excluded for clarity, and can be viewed in Additional file 7: Figure S7.