Figure 3

Developmental and biochemical responses of Arabidopsis Col-0 and Atpht1 knock-out seedlings to Pi limitation on solid medium. Seedlings were grown for 5 d on solid medium containing 250 μM Pi before transfer to fresh medium containing either 250 μM Pi (High Pi) or 5 μM Pi (Low Pi) for 12 d. Root-to-shoot ratio (A), primary root length (B), lateral root length (C), root Pi concentration (D), shoot Pi concentration (E) and anthocyanin concentration (F) were determined at harvest. Values are means ± S.D. (n = 3 plates of 12 seedlings each). * or different letters indicate significantly different means (P <0.05) compared to the corresponding wild-type or each other according to two-way ANOVA and Tukey’s Multiple Comparison of Means, respectively.