Figure 2

Altered plant growth and size of knockout and overexpression mutants of pPLAIIIδ . (A) Morphology of 30-day-old Arabidopsis plants. The size of the KO was slightly enlarged, whereas overexpression of pPLAIIIδ resulted in smaller plants with more compact leaves and shorter petioles. Bar = 1 cm. (B) Individual rosette leaves of 30-day-old plants. The size and the number of rosette leaves of two independent overexpression lines were clearly distinguishable from the WT. From left to right, the leaves were arranged from cotyledons to the youngest rosette leaves. Bar = 1 cm. (C) Aerial parts of WT and OE1. WT was clearly taller than OE. (D) Cross-sections of the stalks of 6-week-old plants stained with toluidine blue. c, cortex; e, epidermis; i, interfascicular cells; ph, phloem; pi, pith; x, xylem. Bars = 100 μm. (E) Morphology of flowers and floral organs. The exposed stigma, smaller flower, altered shape of petal and calyx, and shorter stamen in two OE lines are shown. Inflorescence, flower, stigma, long stamen, short stamen, petal, and calyx are shown from top to bottom. Bar = 3.5 cm. (F) Immature siliques of 50-day-old plants and seeds in mature siliques. The siliques of KO plants were slightly longer, whereas those of all OEs were shorter than those of WT plants. The arrangements of seeds in OEs were crowded, and abortions of ovules in OE2 could be observed (arrows). Bar = 100 μm.