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Table 1 Browning-related genes induced or repressed in the affected inner cortex of ‘Braeburn’ apples

From: Transcriptomic events associated with internal browning of apple during postharvest storage

    

RPKM

 

Malus ID

Gene description

Function

p-value

Healthy

Affected

Fold change

Energy-related pathways

MDP0000192364

Pyruvate dehydrogenase

Link glycolysis to TCA

<0.001

14.53

29.39

2.02

MDP0000129305

Phosphoglycerate mutase

Glycolysis/dually targeted

0.016

0.91

1.80

1.98

MDP0000295823

Probable lactate/malate dehydrogenase

Fermentation

<0.001

20.06

30.24

1.51

MDP0000622920

Phosphoenolpyruvate carboxylase

TCA/org transformation

0.004

8.35

5.47

−1.53

MDP0000198410

Aconitate hydratase

TCA/org transformation

0.016

19.75

11.72

−1.69

MDP0000798440

Cytochrome c biogenesis fn

Mitochondrial electron transport/ATP synthesis

0.014

2.67

1.52

−1.76

Lipid metabolism

MDP0000249250

Phospholipase a2

Lipid degradation

0.004

0.49

4.25

8.65

MDP0000576682*

Butyrate-- ligase peroxisomal-like

FA synthesis and FA elongation

0.01

0.20

1.30

6.63

MDP0000794484

Alpha/beta-Hydrolase

Lipid degradation

0.003

2.60

6.33

2.43

MDP0000833444

Diacylglycerol kinase-like

Phospholipid synthesis

0.018

2.14

5.15

2.41

MDP0000309977

Acyl-coenzyme a thioesterase

Lipid degradation/beta-oxidation

0.004

0.95

2.23

2.36

MDP0000849585

Alpha/beta-Hydrolase

Lipid degradation

0.009

2.85

5.69

2.00

MDP0000283158

Alpha beta-hydrolase, lipase

Lipid degradation

<0.001

4.51

7.92

1.76

MDP0000273425

Diacylglycerol kinase-like

Phospholipid synthesis

0.04

4.44

2.73

−1.63

Redox state

MDP0000615196

Glutaredoxin family protein

Redox state

<0.001

0.32

1.18

3.64

MDP0000146621

Nadh-cytochrome b5 reductase

Redox state

0.004

4.39

8.03

1.83

MDP0000196554

Chorismate mutase/APX

Aminoacid synthesis/ascorbate metabolism

<0.001

11.63

19.96

1.72

MDP0000251669

Thioredoxin

Redox state

0.019

18.00

28.15

1.56

MDP0000252195

Ferredoxin-thioredoxin reductase

Redox state

<0.001

10.84

16.70

1.54

MDP0000520089*

Desacetoxyvindoline 4-

Ascorbate and glutathione metabolism

0.041

5.41

3.53

−1.53

MDP0000181414

Alpha-ketoglutarate-dependent dioxygenase

Redox state

0.004

6.69

4.16

−1.61

Secondary metabolism

MDP0000260512

4-coumarate: ligase

Phenylpropanoids

0.001

0.16

1.05

6.71

MDP0000576682*

Butyrate-- ligase peroxisomal-like

Sulfur-containing glucosinolates synthesis

0.01

0.20

1.30

6.63

MDP0000206680

Reticuline oxidase-like

Alkaloid-like

<0.001

1.48

5.13

3.45

MDP0000702557*

UDP-glucuronosyl/UDP-glucosyltransferase

Flavonoids/flavonols synthesis

0.001

30.37

48.24

1.59

MDP0000520089*

Desacetoxyvindoline 4-

Sulfur-containing glucosinolates synthesis

0.041

5.41

3.53

−1.53

Cell wall

MDP0000610961

L-ascorbate oxidase

Cell wall modifications

<0.001

30.35

132.43

4.36

MDP0000873667

Xyloglucan endotransglucosylase/hydrolase

Cell wall modification

<0.001

52.59

229.40

4.36

MDP0000904458

Fasciclin-like arabinogalactan

Cell wall proteins

<0.001

193.78

525.70

2.71

MDP0000723275

Arabinose 5-phosphate isomerase

Cell wall precursor synthesis

<0.001

18.80

32.91

1.75

MDP0000289339

Cellulose synthase

Cell wall cellulose synthesis

0.013

1.90

1.03

−1.85

MDP0000836165

Pectin methylesterase inhibitor

Cell wall pectin esterases

0.036

2.42

1.21

−2.00

MDP0000616949

Pectin methylesterase inhibitor

Cell wall pectin esterases

0.01

1.04

0.44

−2.37

  1. P-values for DEGs between healthy and affected apples were calculated using PLS-DA, where gene expression values (RPKM) were used as predictor variables and the two class distinctions as response variables. Blast2GO and Mercator web tools were used for gene description and gene function analysis.
  2. *Genes that have been assigned to more than one metabolic pathways.