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Table 1 Germline transmission of T1 mutations to segregated nontransgenic T2 plants

From: A CRISPR/Cas9 toolkit for multiplex genome editing in plants

T1 lines

Nontransgenic T2 plants

NT/Total-T2

ttcc/NT

ttccee/NT

A1

13/62 (21.0%)

6/13 (46.2%)

0

A17

17/74 (23.0%)

17/17 (100%)

9/17 (52.9%)

A25

23/91 (25.2%)

19/23 (82.6%)

0

A32

16/86 (18.6%)

16/16 (100%)

0

A33

12/51 (23.5%)

12/12 (100%)

3/12 (25.0%)

  1. NT, nontransgenic plants; Total-T2, total number of T2 plants examined. ttcc and ttccee correspond to try cpc double and try cpc etc2 triple mutants, respectively.