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Table 1 Germline transmission of T1 mutations to segregated nontransgenic T2 plants

From: A CRISPR/Cas9 toolkit for multiplex genome editing in plants

T1 lines Nontransgenic T2 plants
NT/Total-T2 ttcc/NT ttccee/NT
A1 13/62 (21.0%) 6/13 (46.2%) 0
A17 17/74 (23.0%) 17/17 (100%) 9/17 (52.9%)
A25 23/91 (25.2%) 19/23 (82.6%) 0
A32 16/86 (18.6%) 16/16 (100%) 0
A33 12/51 (23.5%) 12/12 (100%) 3/12 (25.0%)
  1. NT, nontransgenic plants; Total-T2, total number of T2 plants examined. ttcc and ttccee correspond to try cpc double and try cpc etc2 triple mutants, respectively.