Figure 2

Wounding and FACs are both required for systemic JA accumulation. a JA levels in local leaves 0 after wounding. The leaves 0 were wounded with a pattern wheel and 20 μl of water were immediately applied to wounds (W + W), and the JA contents were analyzed. b JA and JA-Ile contents in local and systemic leaves 90 min after W + W treatment. c and d JA and JA-Ile values in different leaves 90 min after applying FAC or FAC-free OS to wounds. Leaves 0 were wounded with a pattern wheel and 20 μl of FAC (27.6 ng/μl; W + FAC) or 20 μl FAC-free M. sexta OS (W + FAC-free OS) were immediately applied to wounds. e JA contents in different leaves 90 min after pressure infiltration of 100 μl FAC (27.6 ng/μl) into leaves 0. Values are mean ± SE; N = 5; n.d. = not detectable.