Figure 6

Full length CgPI and CgAG transcript isolation and multiple sequence alignment of deduced amino acid sequences. (A) Amplification of full length CgPI and CgAG transcripts from total RNA harvested from flower buds. (B) Comparison of CgPI with other PIISTILLATA-like genes. (C) Comparison of CgAG with other AGAMOUS-like genes. Conserved regions are shaded in black. At_PI, Cg_PI, Cs_CUM26 and Cm_pMADS2 are PISTILLATA like genes from Arabidopsis thaliana, Coccinia grandis, Cucumis sativus and Cucumis melo respectively. Cg_AG, Cs_MADS1, Cm_AGAMOUS, Mc_MADS_box2, At_AGAMOUS are AGAMOUS like genes from Coccinia grandis, Cucumis sativus, Cucumis melo, Momordica charantia and Arabidopsis thaliana respectively. MADS domain and K-box are identified by NCBI’s conserved domain database and marked accordingly.