The OsSec18 protein interacts with the Os60sP0 protein both
A, The OsSec18 protein complex in rice endosperm. Crude protein extract from rice immature endosperm was loaded on a Sepharose 300 gel filtration column and detected via Western blotting using anti-Sec18 serum; B, Yeast two-hybrid analysis of OsSec18 and Os60sP0p. Positive, co-transformation with the positive plasmids pGBKT7-53p and GADT7-RecT; negative, co-transformation with the negative plasmids pGBKT7-Lam and GADT7-RecT; C, The Co-IP results using serum against OsSec18 or Os60sP0. The negative control is the antibody against OsSec18 or Os60sP0p in RIPA buffer in the absence of the crude protein extract; D, The tissue-specific expression patterns of the OsSec18 protein. R, root; ST, stem; L, leaf; IF, inflorescence; IMS, immature seed; MS, mature seed.