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Figure 4 | BMC Plant Biology

Figure 4

From: The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell

Figure 4

The OsSec18 protein interacts with the Os60sP0 protein both in vitro and in vivo. A, The OsSec18 protein complex in rice endosperm. Crude protein extract from rice immature endosperm was loaded on a Sepharose 300 gel filtration column and detected via Western blotting using anti-Sec18 serum; B, Yeast two-hybrid analysis of OsSec18 and Os60sP0p. Positive, co-transformation with the positive plasmids pGBKT7-53p and GADT7-RecT; negative, co-transformation with the negative plasmids pGBKT7-Lam and GADT7-RecT; C, The Co-IP results using serum against OsSec18 or Os60sP0. The negative control is the antibody against OsSec18 or Os60sP0p in RIPA buffer in the absence of the crude protein extract; D, The tissue-specific expression patterns of the OsSec18 protein. R, root; ST, stem; L, leaf; IF, inflorescence; IMS, immature seed; MS, mature seed.

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