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Table 2 Protein interactions in yeast

From: Characterization of TM8, a MADS-box gene expressed in tomato flowers

  -W-L a -W-L-H +2 mM 3AT b -W-L-H +5 mM 3AT c -W-L-A d
TM8-BD/AD ++ -- -- --
TM8-BD/TM8-AD ++ -- -- --
BD/TM8-AD ++ -- -- --
TM8-BD/MC-AD ++ ++ ++ ++
BD/MC-AD ++ -- -- --
MC-BD/TM8-AD ++ ++ + +
MC-BD/AD ++ -- -- --
TM8-BD/TM6-AD ++ -- -- --
BD/TM6-AD ++ -- -- --
TM6-BD/TM8-AD ++ -- -- --
TM6-BD/AD ++ -- -- --
TM8-BD/TAP3-AD ++ -- -- --
BD/TAP3-AD ++ -- -- --
TAP3-BD/TM8-AD ++ -- -- --
TAP3-BD/AD ++ -- -- --
TM8-BD/SlGLO1-AD ++ -- -- --
BD/SlGLO1-AD ++ -- -- --
SlGLO1-BD/TM8-AD ++ -- -- --
SlGLO1-BD/AD ++ -- -- --
TM8-BD/SlGLO2-AD ++ -- -- --
BD/SlGLO2-AD ++ -- -- --
SlGLO2-BD/TM8-AD ++ -- -- --
SlGLO2-BD/AD ++ -- -- --
TM8-BD/TAG-AD ++ -- -- +
BD/TAG-AD ++ -- -- --
TAG-BD/TM8-AD ++ -- -- --
TAG-BD/TM8-AD ++ -- -- --
TM8-AD/TAGL1-BD ++ -- -- --
AD/ TAGL1-BD ++ -- -- --
TM8-BD/TM5-AD ++ -- -- --
BD/TM5-AD ++ -- -- --
TM5-BD/TM8-AD ++ -- -- --
TM5-BD/AD ++ -- -- --
TM-8BD/TM29-AD ++ -- -- --
BD/TM29-AD ++ -- -- --
TM-29BD/TM8-AD ++ -- -- --
TM-29BD/AD ++ -- -- --
TM8-BD/SlMBP21-AD ++ -- -- --
BD/SlMBP21-AD ++ -- -- --
SlMBP21-BD/TM8-AD ++ -- -- --
SlMBP21-BD/AD ++ -- -- --
TM8BD/J-AD ++ -- -- --
8BD/J-AD ++ -- -- --
  1. aMedia lacking either adenine or histidine (supplemented with 3-AT, a competitive inhibitor of the HIS3 enzyme.
  2. b-W-L YSD media lacking tryptophan and leucine.
  3. cW-L – H YSD media lacking tryptophan, leucine and histidine.
  4. d-W-L – A YSD media lacking tryptophan, leucine and adenine.
  5. TM8 has been tested for its ability to form dimers with several tomato MADS-box proteins. Interactions have been assayed using TM8 both as bait and as prey. All bait and prey chimeric proteins have been controlled, then they have been transformed with the corresponding empty vector in order to exclude auto-activation problems. Yeast transformations have been repeated twice and each time at least four yeast colonies containing a bait and prey plasmids have been tested for their ability to grow in selective media.