Figure 6
The effect of exogenous amino acid treatments on Fusarium susceptibility in Arabidopsis floral, silique and rosette leaf tissue. Arabidopsis plants of genotype eds1-2 were sprayed at early flowering with either 10 mM D-homoserine (DHS), L-homoserine (LHS), threonine (THR) or sterile water, co-incident with F. culmorum. Amino acid/water treatments were then repeated daily for 6 dpi. Disease was assessed at 7 dpi. a) Images show infected apical inflorescences (upper panel) and rosette leaves (lower panel) – stem and floral tissue have been removed from rosettes. White arrow – green and opening buds present in LHS treated plants. Plants were scored for (b) bud disease, (c) open flower disease, (d) silique disease and (e) infected rosette leaf number. Asterisks indicate statistically significant differences from H2O treated plants (regression analysis followed by prediction of LSDs, p = <0.05, n = 8). Results are representative of two independent experiments.