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Figure 7 | BMC Plant Biology

Figure 7

From: Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium

Figure 7

Cold-temperature treatment of G. hirsutum seedlings. Plants were grown in a growth chamber at 30°C for 13 days, then a portion of the plants were transferred at the beginning of the 14th day to a similar chamber held at 10°C and the first true leaves were sampled for 24 hours for fatty acid and gene expression analysis. Images of plants grown at either 30°C (Control) (A) or 10°C (Cold-treated) (B) for 6 hours showed significant wilting of plant leaves at 10°C (B). Fatty acid composition of control (C) or cold-treated (D) plants was determined over a 24-hour period. Values represent the average and standard deviation of three biological replicates. Student’s t-test was used to compare percentages of each fatty acid between control and cold-treated samples. Solid, upward pointing arrowheads in panel (D) represent a statistically significant increase in fatty acid composition (p <0.05) in response to cold, while down arrowheads represent a decrease in response to cold (p <0.05). The inset in panel (D) shows a line graph of 18:3 fatty acid content at either 30 or 10°C (*, p <0.05). (E) Representative semi-quantitative RT-PCR analysis showing prominent cold-induced expression of the FAD7/8-1 gene at 10°C (right side) compared to the 30°C control samples (left side). (F) Quantitative analysis of band intensities in panel (E) relative to t = 0 for the same temperature treatment revealed a statistically significant induction of FAD7/8-1A expression at 10°C (open circles, dashed line) in comparison to 30°C (closed circles, dashed line), while FAD7/8-2A was not induced by cold temperature (open squares, solid line) in comparison to the control (closed squares, solid line). Values represent the average and standard deviation of three biological replicates, and student’s t-test was used for comparison of the same gene at different temperatures. * denotes p <0.05.

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