Figure 4

Quantitative RT-PCR analysis of CPD and DWF4 transcript levels. Plants were grown and harvested as described in Figure 1. A, Relative CPD transcript levels in wild-type (C24) and cbb1 plants. B, Relative DWF4 transcript levels in wild-type, CPD-antisense, and cbb1 plants. C, Relative CPD transcript levels in BRZ treated plants. D, Relative DWF4 transcript levels in BRZ treated plants. eIF1a CT values were subtracted from respective CT values of the gene of interest resulting in dCT. Subsequently, differences were subtracted from an arbitrary value (i.e. 40). Higher numbers indicate higher transcript levels. A difference of one unit indicates a fold change of two. Data are given as mean ± SE of gene of interest in three technical replicates. The data shown are from one experiment representative of three independent biological replicates.