Figure 5
From: Increased sucrose levels mediate selective mRNA translation in Arabidopsis
Real-time qRT-PCR confirmation of the results obtained by microarray analysis for samples treated with sucrose in the light. A) Comparison of the RNA distribution in non-polysomal (NP, light color) and polysomal (PL, dark color) fractions after performing sucrose gradient fractionation without prior cushion purification. Data was normalized on LUC spike-in mRNA and the sum of signal in NP and PL was set as 100%. Bars show averages of 3 independent biological replicates ± SD. Asterisk denotes significant differences between sucrose and control as determined by Student's t-test (p < 0.05). B) Real-time qRT-PCR analysis of gradient fractions of gradients performed after sucrose cushion enrichment of polysomes from the top (fraction 1) to the bottom of the gradient (fraction 12). Shown are averages of three independent biological replicates ± SD, expression values normalized on LUC spike and on the total area under the curve in the corresponding polysomal gradient analysis.