Figure 7
Plant materials. (A) Appearance of stem cuttings in clones 25-5 and 23-12. On 14 DAC, adventitious root cells began to dedifferentiate and divide, white callus (arrow) formed in the region between the xylem and epidermis at the base of cuttings in clone 25-5. Then, on 25 DAC, white protrusions (arrow), which later developed into adventitious roots, were located around callus after adventitious root initial cell formation. More white protrusions formed in clone 25-5 than in 23-12. By 35 DAC, young roots (arrow) protruded through the epidermis and began to elongate with the emergence and development of adventitious root primordia. More young roots formed in clone 25-5 than in clone 23-12. (B) Part of stem cutting used in this study. Adventitious roots formed at the bottom section (5-mm thick; arrow) of cuttings. Therefore, proteins and RNA were extracted from this part for analysis in this study. DAC: days after cutting.