Quantitative (q)PCR analysis of genes differentially regulated in response to inactivation of SnRK1. Values were determined by qPCR analysis of total RNA isolated from Arabidopsis leaves infused with Agrobacterium containing DNA capable of expressing full-length Spinach curly top virus C2, antisense (as)SnRK1.2, an inverted repeat construct designed to express dsRNA (dsSnRK1.2) or an empty plasmid vector (pMON530). The columns represent relative mRNA levels in C2, asSnRK1, or dsSnRK1-infused leaves as compared to levels present in leaves infused with Agrobacterium containing empty plasmid vector (pMON530), which was arbitrarily assigned a value of 1 at each time point. The fold change was calculated from the mean ΔΔCt values from three independent experiments using RNA isolated two days post-infusion (dpi). Error bars represent the Standard Error of the mean and asterisks indicate significant differences in expression as determined using the Student's t-test (P < 0.05) on ΔCt values.