Table 1 Primers used to recover partial and complete PnTgs1-like sequences from apomictic and sexual genotypes (fragments F1, F2, F3, F4)

F1 forward

5′-AAACCCAAACGGCTAAAACC-3′

60.2°C

F1 reverse

5′-CCTTGACGTGTCCTCAGACTCTAGC-3′

64.9°C

F2 forward

5′-TCTGCTGAAAAAGCCCCTGGC-3′

51.2°C

F2 reverse

5′-CCTCAACCGCCCATGGAGGA-3′

66°C

F3 forward

5′-CAGGAGTTACAGATTTGGCCCTTGC-3′

67.8°C

F3 reverse

5′-GGGGTGGACCTGACTATGCTAAAG-3′

66.3°C

F4 forward

5′-AAACCCAAACGGCTAAAACC-3′

60.2°C

F4 reverse

5′-GGGGTGGACCTGACTATGCTAAAG-3′

66.3°C