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Table 1 Primers used to recover partial and complete PnTgs1-like sequences from apomictic and sexual genotypes (fragments F1, F2, F3, F4)

From: PnTgs1-like expression during reproductive development supports a role for RNA methyltransferases in the aposporous pathway

Primer Sequence Tm (°C)
F1 forward 5′-AAACCCAAACGGCTAAAACC-3′ 60.2°C
F1 reverse 5′-CCTTGACGTGTCCTCAGACTCTAGC-3′ 64.9°C
F2 forward 5′-TCTGCTGAAAAAGCCCCTGGC-3′ 51.2°C
F2 reverse 5′-CCTCAACCGCCCATGGAGGA-3′ 66°C
F3 forward 5′-CAGGAGTTACAGATTTGGCCCTTGC-3′ 67.8°C
F3 reverse 5′-GGGGTGGACCTGACTATGCTAAAG-3′ 66.3°C
F4 forward 5′-AAACCCAAACGGCTAAAACC-3′ 60.2°C
F4 reverse 5′-GGGGTGGACCTGACTATGCTAAAG-3′ 66.3°C