In situ expression of PnTgs1-like in reproductive tissues of sexual (Q4188) and apomictic (Q4117) Paspalum notatum genotypes at anthesis. Sexual genotype Q4188 ovaries hybridized with an antisense PnTgs1-like RNA probe, showing an intense signal in nucella and integuments (A, B, C). Larger images of ovules originated from sexual genotype Q4188 (D, E, F). Apomictic genotype’s (Q4117) ovaries displaying a lower expression of PnTgs1-like and showing several non-reduced embryo sacs within the ovule (G, H, I). Details of embryo sacs originated from apomictic genotype Q4117 (J, K, L). White dotted lines indicate ovule boundaries. OV: ovule; PN: polar nuclei; EA: egg apparatus. Bars: 100 μm.