Cysteine protease and cystatin expression and activity during soybean nodule development and senescence

BMC Plant Biology

Table 2 Expression and inhibitory potency of cystatins against proteases from different aged nodules

Cystatin	Expression	Cat-L inhibition	Cat-B inhibition
Active
4 weeks
Glyma05g28250	+ (22.65)	+ (36.1%)	+ (32.8%)
Glyma13g04250	+ (97.58)	+ (26.4%)	+ (27.6%)
Glyma14g04250	(−) (2.14)	(−)	(−)
Glyma15g36180	+ (26.34)	++ (49.9%)	++ (48.7%)
Glyma20g08800	+ (85.83)	(−)	(−)
14 weeks
Glyma05g28250	+ (39.78)	+ (30.6%)	(−)
Glyma13g04250	+ (63.86)	+ (29.7%)	(+) (24.9%)
Glyma14g04250	(+) (12.38)	(+) (21.9%)	(−)
Glyma15g36180	+ (55.64)	(−)	(−)
Glyma20g08800	+ (56.25)	(−)	(−)
Non-active
4 weeks
Glyma04g10360	(−) (0)	+ (38.6%)	+ (35.3%)
Glyma07g39590	(−) (2.09)	+ (47.5%)	+ (42.3%)
Glyma08g11210	(−) (0)	+ (43.6%)	+ (42.1%)
Glyma18g12240	(−) (0.28)	++ (54.0%)	+ (36.6%)
Glyma13g27980	(−) (0)	+ (33.2%)	+ (42.0%)
14 weeks
Glyma04g10360	(−) (0)	+ (39.0%)	+ (28.6%)
Glyma07g39590	(−) (1.23)	++ (51.3%)	+ (34.0%)
Glyma08g11210	(−) (0)	+ (33.5%)	(−)
Glyma18g12240	(−) (0.58)	++ (51.5%)	(+) (22.4%)
Glyma13g27980	(−) (0)	(−)	(−)

++ strong, + medium. (+) low and (−) no cystatin expression/or activity (tested up to 1 mM). Expression indicated as measured FPKM abundances and activity indicated as% inhibition.

ISSN: 1471-2229