HSI2/VAL1 PHD-like domain promotes H3K27 trimethylation to repress the expression of seed maturation genes and complex transgenes in Arabidopsis seedlings

Table 1 Estimation of LUC copy number in Arabidopsis plants containing either Kan ^S or Kan ^R locus or both

Genotype	Chromosomal locations	CalculatedLUCcopy number	EstimatedLUCcopy number
Kan ^S	5	2.00 ± 0.33	2
Kan ^R	4	4.17 ± 0.47	5
WT^LUC	-	7.10 ± 0.33	7

Number of LUC copies was determined by absolute quantitative real-time PCR. Calibration curves were created using pBI121-GSTF8::LUC plasmid DNA as a template. A single copy gene, At5g47480, was used as an internal control for normalization of the data. WT^LUC plants contain two unlinked transgene insertion loci composed of multiple T-DNA insertions. While both insertions express LUC only one expresses NPTII and confers resistance to kanamycin. These loci were separated by genetic segregation to produce lines that are kanamycin sensitive (Kan^S ) or kanamycin resistant (Kan^R). LUC copy numbers were calculated for the Kan^S, Kan^R and WT^LUC Arabidopsis lines as described previously [32],[33]. Genomic DNA from Col-0 wild-type plants was used as a negative control.

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