Figure 7

ChIP-qPCR analyses of H3K27me3 levels on the master transcriptional regulators of seed-maturation program and seed maturation related target genes of HSI2 PHD-like domain in WT^LUCand hsi2^LUCmutant seedlings. Chromatin samples were prepared using five day old WT^LUC, hsi2-2^LUC and hsi2-4^LUC seedlings. Immunoprecipitations were performed using either IgG (non-specific binding control) or anti-H3K27me3 antibody. Data represent means (’sD) obtained from three qPCR reactions each from three independent immunoprecipitations from three different biological replicates. Significant differences in H3K27me3 enrichments between WT^LUC and hsi2^LUC mutants (WT^LUC versus hsi2-2^LUC, WT^LUC versus hsi2-4^LUC) are indicated by letters (a = p < 0.05, b = p < 0.005, c = p < 0.0005, d = p < 0.0001). ABSCISIC ACID-INSENSITIVE3 (ABI3, At3g24650), FUSCA3 (FUS3, At3g26790), LEAFY COTYLEDON1 (LEC1, At1g21970), LEAFY COTYLEDON2 (LEC2, At1g28300), LEC1-LIKE (L1L, At5g47670), AGAMOUS-Like 15 (AGL15, At5g13790), At2g41260 (Late-embryogenesis-abundant protein), At3g22640 (cupin family seed storage protein), At1g04660 (glycine-rich protein), At2g29370 (NAD(P)-binding Rossmann-fold superfamily protein), At5g45830 (DOG1, DELAY OF GERMINATION1), At2g34700 (putative proline-rich glycoprotein).