Figure 6

ChIP-qPCR analyses of H3K4me3, H3K9me2, H3K27me3 and H3K36me3 enrichments on endogenous GSTF8 promoter and GSTF8::LUC transgene regions in WT^LUCand hsi2-4 carrying either Kan^Sor Kan^Rlocus. Chromatin samples were prepared using five day old seedlings from various genotypes. ChIP-qPCR data represents mean values (’sD) of three PCR reactions obtained from each of two independent immunoprecipitations. Significant differences between wild-type lines and corresponding mutant lines (Kan^S versus hsi2-4-Kan^S, Kan^R versus hsi2-4-Kan^R) were determined using two-tailed Student’s t-test assuming unequal variances and P values are indicated by letters (a = p < 0.05, b = p < 0.005, c = p < 0.0005, d = p < 0.0001).