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Figure 5 | BMC Plant Biology

Figure 5

From: HSI2/VAL1 PHD-like domain promotes H3K27 trimethylation to repress the expression of seed maturation genes and complex transgenes in Arabidopsis seedlings

Figure 5

Chromatin immunoprecipitation (ChIP) and quantitative PCR (qPCR) analyses of H3K4me3, H3K9me2, H3K27me3 and H3K36me3 levels on endogenous GSTF8 promoter and transgene chromatin in WTLUCand hsi2-4LUCmutant. A. Genomic structures of endogenous GSTF8 gene and GSTF8::LUC transgene showing the locations of amplified regions by ChIP-qPCR. B. qPCR analyses of chromatin samples from 5-day old seedlings of WTLUC and hsi2-4LUC that were immunoprecipitated using either specific antibodies recognizing indicated histone methylation marks or IgG (non-specific binding control). Data is expressed as percentage of immunoprecipitated DNA relative to input DNA. ACT2/7 (H3K4me3 and H3K36me3), FUS3 (H3K27me3) and TA2 (H3K9me2) serve as positive controls whereas TA2 (H3K4me3 and H3K36me3) and ACT2/7 (H3K9me2 and H3K27me3) were used as negative controls. Data represent means (’sD) from two biological replicates with three qPCR replicates each. Significant differences in enrichment between WTLUC and hsi2-4LUC for each genomic region tested were determined using two-tailed Student’s t-test assuming unequal variances and P values are indicated by letters (a = p < 0.05, b = p < 0.005, c = p < 0.0005, d = p < 0.0001).

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