Figure 1

Genomic structure of GSTF8::LUC transgene and luminescence imaging of WT^LUCand hsi2-4 mutant seedlings harboring either Kan^Ror Kan^Stransgene locus or both. A. GSTF8::LUC transgene contain neomycin phosphotransferase (NPTII) coding sequences under the control of nopaline synthase (NOS) promoter and a modified luciferase (LUC⁺) coding sequences from firefly driven by glutathione S-transferase F8 (GSTF8) promoter conferring kanamycin resistance and luminescence expression respectively in plants. The 3′ ends of both NPTII and LUC⁺ coding sequences include NOS terminator sequences for transcriptional termination. B. Plants harboring either Kan^R or Kan^S transgene locus alone in the wild-type or in hsi2-4 mutant background were obtained by crossing of either WT^LUC or hsi2-4^LUC into Columbia-0 wild-type and homozygous lines were identified in F₂ and F₃ generations. Five days old seedlings of various genotypes grown on Murashige and Skoog media plates were imaged using cooled CCD camera after spraying with the substrate luciferin. Pseudocolor image indicates luminescence intensity from lowest (blue) to highest (white).