Figure 3

cDNA and genomic DNA sequences of AoDHN1. (A) cDNA of 573 bp corresponding to Open Reading Frame (ORF) of AoDHN1 obtained from Rapid Amplification of cDNA Ends (RACE) PCR. Y, S and two of K segments are depicted on the ORF. (B) Genomic fragment of AoDHN1 with intron of 107 bp. (C) Nucleotide sequence of AoDHN1 and its corresponding translated protein sequence. Arrowhead indicates intron location and underline indicates Nuclear Localization Signal (NLS) sequence. (D) Predicted three dimensional structure of AoDHN1 obtained using iTASSER server (http://zhanglab.ccmb.med.umich.edu/I-TASSER/) showing two alpha helices (in red), but the rest of the molecule is unstructured.