Silencing of either SlSR1 or SlSR3L activated constitutive defense response. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-SlSR1, pTRV2-SlSR3L or pTRV2-GUS constructs and leaf samples were collected at 2 weeks after VIGS infiltration for detection of H2O2 accumulation and analysis of expression of signaling- and defense-related genes. (A) Accumulation of H2O2 in leaves of the pTRV2-SlSR1- and pTRV2-SlSR3L-infiltrated plants. (B) Expression of defense-related genes and ROS production-related genes in the pTRV2-SlSR1- and pTRV2-SlSR3L-infiltrated plants. (C) Expression of genes involved in different defense signaling pathways in the pTRV2-SlSR1- and pTRV2-SlSR3L-infiltrated plants. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p?<?0.05 level.