Differential transcriptome analysis reveals insight into monosymmetric corolla development of the crucifer Iberis amara

Table 2 RNA-Seq reads and mapping statistics

Samples	aD_1	aD_2	aB_1	aB_2
Total clean reads	25,793,295	42,131,488	28,273,758	38,725,897
Mapped reads	16,468,215	26,813,097	17,810,445	24,428,816
Mapped uniquely	16,465,583	26,808,334	17,806,437	24,423,583
Mapped non-specifically	2,632	4,763	4,008	5,233
Unmapped reads	9,325,080	15,318,391	10,463,313	14,297,081
Percent mapped uniquely	63.84	63.63	62.98	63.07

All clean reads obtained from the four libraries from adaxial (samples aD_1 and aD_2) and abaxial (samples aB_1 and aB_2) petals of stage A1 Iberis flowers were mapped against the re-assembled contigs. Only reads that map uniquely to one locus, highlighted in bold print, were used for the calculation of gene expression. This applies to 63-64% of total reads from the four libraries.

ISSN: 1471-2229