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Figure 3 | BMC Plant Biology

Figure 3

From: Rice SUV3 is a bidirectional helicase that binds both DNA and RNA

Figure 3

Direction of DNA unwinding by OsSUV3 protein. (A) The structure of the linear substrate for the measurement of 3' to 5' direction activity is shown. An asterisk (*) denotes the 32P-labeled end. The 3' to 5' direction specific DNA helicase activity of OsSUV3 protein using this substrate is shown in panels B and C. (B) Lanes 1-8 are reactions with increasing concentration (10-300 nM) of OsSUV3 protein. (C) Time-dependence of the helicase reaction with substrate shown. Lanes 1-7 are reactions with 250 nM of OsSUV3 at various time points. (D) The structures of the linear substrate for the 5' to 3' direction is shown. An asterisk (*) denotes the 32P-labeled end. The 5' to 3' direction specific DNA helicase activity of OsSUV3 protein using this substrate is shown in panels E and F. (E) Lanes 1-7 are reactions with increasing concentration (30-300 nM) of OsSUV3 protein. (F) Time-dependence of the helicase reaction with substrate shown. Lanes 1-6 are reactions with 250 nM of OsSUV3 at various time points written on top of the autoradiogram. The quantitative enzyme activity data from the autoradiogram in B-C and E-F are shown as histograms on the right side. In panels B, C, E and F, lane C is the reaction without enzyme and lane B is the heat-denatured substrate.

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