Figure 5

Differential gene expression of ZmLTPs under drought stress. (A) Expression profiles of 36 ZmLTPs under moderate drought stress (M/C), severe drought stress (S/C) and re-watering (R/C) as compared to control seedlings in Han21 and Ye478, respectively. Log2-based fold changes were used to create the heatmap. (B) Venn diagrams for the number of differentially expressed ZmLTP genes under moderate drought stress (M/C), severe drought stress (S/C) and re-watering (R/C) as compared to control seedlings in Han 21 and Ye478, respectively. A gene is considered differentially expressed if it is up- or down-regulated at least two-fold with a P value <0.05 under any of the given conditions. (C) Quantitative RT-PCR analysis for selected ZmLTP genes in drought treatments. The maize UBQ1 gene was used as endogenous control to normalize data. The values represented are the mean of two biological replicates, each with three technical replicates. Error bars represent standard deviations determined with two biological replicates.