Figure 2

The structure features of maize nsLTPs. (A) Sequence alignment of maize ZmLTP1.6 (1FK7; amino acids (aa) 1-93), wheat nsLTP2 (1TUK; aa 1-67), AtDIR1 (2RKN; aa 1-77), ZmLTP1.2 (aa 29-121), ZmLTP2.1 (aa 32-98) and ZmLTPd5 (aa 37-115). Identical residues are highlighted by blue background. Consensus residues Thr-X-X-Asp-Arg and Pro-Tyr-X-Ile-Ser are marked in boxes. (B) Schematic representation of the cystein pairing pattern and superposition of the backbone trace of ZmLTP1.2 (blue) and ZmLTP1.6 (grey) complexed with ricinoleic acid (shown as ball-and-stick; carbons in yellow and oxygen in red). The four disulfide bonds are shown in orange. (C) Schematic representation of the cystein pairing pattern and superposition of the backbone trace of ZmLTP2.1 (pink) and nsLTP2 (grey) complexed with LPG (L-α-palmitoyl glycerol) lipid ligands (shown as ball-and-stick; carbons in yellow and oxygen in red). The four disulfide bonds are shown in orange. (D) Schematic representation of the cystein pairing pattern and superposition of the backbone trace of ZmLTPd5 (cyan) and the crystal structure of AtDIR1 (grey) in complex with two lysophosphatidyl choline molecules (shown as ball-and-stick; carbons in yellow and oxygen in red.). The four disulfide bonds are shown in orange. (E) ERRAT result depicting overall quality factor and Ramachandran plot of ZmLTP1.2, ZmLTP2.1 and ZmLTPd5.