Relative levels of scopolin and scopoletin in the roots of seven Arabidopsis thaliana accessions. (A) Scopolin level in methanol root extracts without hydrolysis (H-). (B) Scopoletin content in the methanol extracts that were subjected to enzymatic hydrolysis (H+) prior to quantification. The statistically significant differences between group means for scopolin and scopoletin accumulation were determined by one-way ANOVA (p < 0.001 and p < 0.0001, respectively). Values that are not significantly different based on the post hoc test (least significant differences [LSD]) are indicated by the same letters. The data analysis consisted of scopolin and scopoletin relative levels measured as area% of total chromatogram signals. Error bars represent the SD from three measurements.