Figure 2

GC- MS analysis of sesquiterpenes (A), monoterpenes and diterpenes (B) produced by recombinant PtTPS5, PtTPS6, PtTPS7, PtTPS9, PtTPS10, PtTPS11, PtTPS12, PtTPS13 and PtTPS15. The enzymes were expressed in E. coli, extracted, partially purified, and incubated with the substrates FPP, GPP and GGPP. Products were collected with a solid-phase microextraction (SPME) fiber and analyzed by GC-MS. 1, elemol; 2, β-eudesmol*; 3, unidentified sesquiterpene alcohol; 4, (E)-β-caryophyllene*; 5, β-humulene*; 6, β-elemene*; 7, eremophilene; 8, β-selinene*; 9, unidentified sesquiterpene; 10, β-curcumene*; 11, nerolidol*; 12, (E)-β-ocimene*; 13, myrcene*; 14, limonene*; 15, terpinolene*; 16, linalool*; 17, sabinene*; 18, 1,8-cineole*; 19, terpineol*; 20, geranyllinalool*; cont., contamination. Compounds marked with * were identified using authentic standards.