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Table 2 Construct design for enzymatic assays in E. coli

From: Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits

Genes to be analysed Plasmids contained in E. coli (BL21) and the carotenoid abeing produced (in parentheses)
Negative control Functional assays Positive control
LbPSY1/LrPSY1 pACCRT-E + pEASY-E1 pACCRT-E + pEASY-LbPSY1 or pACCRT-E + pEASY-LrPSY1 pACCRT-EB
(GGPP) (phytoene)
LbCYC-B/LrCYC-B pACCRT-EIB + pEASY-E1 pACCRT-EIB + pEAST-LbCYC-B or pACCRT-EIB + pEASY-LrCYC-B pACCAR16ΔcrtX
(lycopene) (β-carotene)
LbCRTR-B2/LrCRTR-B2 pACCAR16ΔcrtX + pEASY-E1 pACCAR16ΔcrtX + pEASY-LbCRTR-B2 or pACCAR16ΔcrtX + pEASY-LrCRTR-B2 pACCAR25ΔcrtX
(β-carotene) (zeaxanthin)
  1. aGGPP is an exception.