Table 2 Construct design for enzymatic assays in E. coli
Genes to be analysed | Plasmids contained in E. coli (BL21) and the carotenoid abeing produced (in parentheses) | ||
|---|---|---|---|
Negative control | Functional assays | Positive control | |
LbPSY1/LrPSY1 | pACCRT-E + pEASY-E1 | pACCRT-E + pEASY-LbPSY1 or pACCRT-E + pEASY-LrPSY1 | pACCRT-EB |
(GGPP) | (phytoene) | ||
LbCYC-B/LrCYC-B | pACCRT-EIB + pEASY-E1 | pACCRT-EIB + pEAST-LbCYC-B or pACCRT-EIB + pEASY-LrCYC-B | pACCAR16ΔcrtX |
(lycopene) | (β-carotene) | ||
LbCRTR-B2/LrCRTR-B2 | pACCAR16ΔcrtX + pEASY-E1 | pACCAR16ΔcrtX + pEASY-LbCRTR-B2 or pACCAR16ΔcrtX + pEASY-LrCRTR-B2 | pACCAR25ΔcrtX |
(β-carotene) | (zeaxanthin) | ||