Figure 5
From: Accumulation and cellular toxicity of aluminum in seedling of Pinus massoniana
Effects of different concentrations of Al on spindle MTs in mitosis in root tip cells of P. massoniana . DNA staining with DAPI (A1-D1, blue), tubulin immunolabeling (A2-D2, green), merged images (A3-D3) and bright field images (A4-D4) in the same single optical section obtained with the confocal laser scanning microscope. Bar = 10 μm for all figures. A. Showing anaphase chromosome and spindles in control cell. B. Showing sticky spindle and MT fragments in anaphase (10-4 M Al, 24 h). C. Showing depolymerized spindle MTs and chromosome stickiness (10-3 M Al, 48 h). D. Showing normal phragmoplast (Control). E. Showing depolymerized phragmoplast (10-3 M Al, 48 h).